A Micro-Method Developed for Prothrombin Time Assay
نویسندگان
چکیده
منابع مشابه
Determination of prothrombin by a micro-coagulation method.
In contrast to earlier assays, the prothrombin determination described here uses a two-step reaction: first, prothrombin is activated with human factor Xa, phospholipids, Ca++ and factor V; second, the amount of activated prothrombin is assayed by the proteolysis of added fibrinogen. The assay is dependent only on prothrombin, and independent of other components of thrombin generation. Carboxy-...
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Prothrombin time (PT) is the leading test for monitoring oral anticoagulation therapy (OAT). We sought to determine INR taking into account only active coagulation factors FII, FVII and FX without inhibition in patient plasmas and calibrator kits.We measured PT using a combined thromboplastin reagent. The calculation was based on a new PT method, which measures active coagulation factors (F II,...
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Background: Psoriasis is a common chronic and recurrent inflammatory skin disorder related to the immune system and characterized by the rapid growth of skin cells. To date, no study has been conducted on the role of the prothrombin time index in determining the disease activity and the prognosis of these patients. Recognizing the factors influencing the prognosis of this disease can be helpf...
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A new method for the specific assay of prothrombin is described which utilises Taipan snake venom and the chromogenic substrate Tos-Gly-Pro-Arg-pNA (Chromozym TH). The method is sensitive and reproducible and shows good correlation with a conventional specific assay for prothrombin. The reaction is dependent on calcium ions and phospholipid, and is therefore sensitive to the coumarin-induced pr...
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Recent advances in physiology Dam (1934; 1935) observed that chicks on synthetic diets developed haemorrhages uncontrollable by vitamins A, C and D. He therefore attributed the bleeding tendency to lack of a specific food factor, vitamin K (coagulation vitamin). Dam and Schonheyder (1934) demonstrated that the delay in coagulation in chicks on diets deficient in vitamin K was not caused by a di...
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ژورنال
عنوان ژورنال: Experimental Animals
سال: 1978
ISSN: 0007-5124,1881-7122
DOI: 10.1538/expanim1978.27.3_311